3D printing of osteocytic Dll4 integrated with PCL for cell fate determination towards osteoblasts in vitro
نویسندگان
چکیده
Since 3D printed hard materials could match the shape of bone, cell survival and fate determination towards osteoblasts in such have become a popular research target. In this study, scaffold material for fabrication was designed to regulate developmental signal (Notch) transduction guiding osteoblast differentiation. We established polycaprolactone (PCL) cell-integrated printing system (PCI3D) reciprocally print beams PCL cell-laden hydrogel module. This PCI3D module holds good viability over 87%, whereas cells show about sixfold proliferation 7-day culture. The osteocytic MLO-Y4 engineered overexpress Notch ligand Dll4, making up 25% after mixing with 75% stromal Osteocytic unlike other delta-like family members as Dll1 or Dll3, promotes differentiation mineralization primary mouse line bone marrow when cultured 28 days. Mechanistically, Dll4 not promote osteogenic (BMSCs) conditional deletion transcription factor RBPjκ by Cre recombinase. These data indicate that activates RBPjκ-dependent canonical signaling BMSCs their oriented osteoblasts. Additionally, great potential angiogenesis human umbilical vein endothelial within modules. Our study reveals be niche determining will open new avenue overcome current limitation poor low bioactivity traditional orthopedic implants.
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ژورنال
عنوان ژورنال: Bio-design and manufacturing
سال: 2022
ISSN: ['2096-5524', '2522-8552']
DOI: https://doi.org/10.1007/s42242-022-00196-1